CRISPR in action: Practice and theory of genome engineering
Date: 17 - 21 August 2026
Duration: P1DT40H
Language of instruction: English
CRISPR technology has revolutionized genome editing, making precise genetic modifications with unprecedented speed and accessibility. For anyone working in molecular biology or genetics, a solid understanding of CRISPR is no longer optional - it's essential. You will learn how to design gRNAs, build CRISPR vectors, perform genome edits, and evaluate editing outcomes. By the end of the course, you will have the knowledge and practical skills to confidently design and execute your own CRISPR experiments, bridging the gap between concept and real-world application. Through interactive lectures and guided laboratory sessions, this hands-on training combines theory and practice to give you a comprehensive introduction to CRISPR-based genome engineering. You can engage with invited experts during their lectures.
Keywords: hands-on, omics
Venue: Heverlee - Landbouwinstituut, Kasteelpark Arenberg 20
City: Leuven
Country: Belgium
Postcode: 3001
Learning objectives:
- “Construct CRISPR vectors applying appropriate cloning strategies and component selection”
- “Design gRNAs using efficiency prediction and assess off-target risk”
- “Explain different approaches to analyzing CRISPR experiments”
- “Identify potential experimental pitfalls and propose improvements to CRISPR workflows”
- “Interpret editing results”
- “Optimize HDR donor templates for precise genome edits according to design criteria”
- “Perform genome editing and conduct genotyping analysis with Sanger sequencing”
Organizer: VIB (https://ror.org/03xrhmk39)
Event types:
- Workshops and courses
Instructors: 
Sibylle Vonesch, Ruben Vazquezuribe, Olaf Klingbeil
Activity log
