NEOF - Introduction to sequencing data and quality control
Date: 28 - 30 October 2025
Timezone: London
Language of instruction: English
There are 3 sequencing technologies that are heavily used for biological research; Illumina, PacBio, and ONT. These three technologies each have their own errors and biases. At the end of this course you will understand these error profiles, and be able to use command line tools to measure sequencing quality and conduct quality control.
Places are restricted to UK-based researchers and only 30 places are available so please only register if you intend on attending.
The workshop will be held over two days on Tuesday 28 and Thursday 30 October 2025, 10am-4pm, with registration opening 9.30am on Tuesday.
The deadline to register is Monday 13 October, 2 weeks before the course. You will hear later that week whether you have been invited and will need to confirm your attendance.
Register to NEOF mailing list to hear first about future NEOF free bioinformatics courses: https://neof.org.uk/news/
City: Online
Country: United Kingdom
Learning objectives:
At the end of this book learners will be able to:
- Explain how sequencing by synthesis works.
- Understand where common Illumina errors come from.
- Quality check Illumina fastq files with fastQC and multiQC.
- Quality control Illumina fastq files by trimming and filtering.
- Explain how ONT & PacBio sequencing works.
- Understand the advantages and disadvantages of both technologies.
- Quality check ONT data with NanoPlot.
- Remove ONT adaptors with Porechop.
- Filter ONT reads by length and quality with Chopper.
- Filter adapters from PacBio data with HiFiAdapterFilt.
- Convert PacBio BAM files to fastq.
- Quality check PacBio CCS reads with RabbitQCPlus.
Organizer: NEOF
Event types:
- Workshops and courses
Sponsors: NERC
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